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. 2016 May 31;7(27):41843–41856. doi: 10.18632/oncotarget.9731

Figure 1. Inhibitory effects of Ary on the growth and colony formation of cervical cancer cells.

Figure 1

(A) Chemical structure of Ary. (B) MTT assay of Hela cells treated with Ary at the indicated concentrations after 24 h. (C) MTT assay of Caski cells treated with Ary at the indicated concentrations after 24 h. The absorbance ratios to the blank control were calculated in MMT results. Data are shown as the mean ± SD of three independent experiments by analysis of Student's t test. *P < 0.05; **P < 0.01. (D) Hela cells were treated with Ary at the indicated concentrations, and then cultured in soft agar for 2 weeks. After crystal violet staining, cell colonies were counted. (a), blank; (b), 1.25 μg/mL; (c), 2.5 μg/mL; (d), the inhibitory rates were calculated. (E) Caski cells were treated with Ary at the indicated concentrations, and then cultured in soft agar for 2 weeks. After crystal violet staining, cell colonies were counted. (a), blank; (b), 1.25 μg/ mL; (c), 2.5 μg/mL; (d), the inhibitory rates were calculated. The inhibitory rates of colony formation were calculated to the blank control. Data are shown as the mean ± SD of three independent experiments by analysis of Student's t test. **P < 0.01.