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. 2016 May 31;7(27):41843–41856. doi: 10.18632/oncotarget.9731

Figure 6. Ary induces cervical carcinoma cell arrest at G1/S through cyclin A2 and Cdk2.

Figure 6

(A) HeLa cells were transfected with siCyclinA2 for 24 h, and then were treated with Ary at 2.5 μg/mL for 24 h. Cyclin A2 was detected in the treated cells with Western-blotting (a), and the abundance ratios of cyclin A2 expressions to β-actin were calculated (b). (B) Cell cycle of the treated cells was analysed by flow cytometry. (a), blank; (b), 2.5 μg/mL; (c), siCyclinA2; (d), siCyclinA2 and Ary. (C) HeLa cells were pretreated with flavopiridol for 24 h, and then were treated with Ary at 2.5 μg/mL for 24 h. Cdk2 was detected in the treated cells with Western-blotting (a), and the abundance ratios of Cdk2 expressions to β-actin were calculated (b). (D) The treated cells were analyzed by flow cytometry. (a), blank; (b), 2.5 μg/mL; (c), flavopiridol; (d), flavopiridol and Ary.