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. 2016 May 26;7(27):41974–41985. doi: 10.18632/oncotarget.9633

Figure 2. The IL-6/STAT3/NF-κB autocrine positive feedback loop is active in activated fibroblasts in vitro and in tumors.

Figure 2

Exponentially growing cells were exposed for 24 h to SFM, SFM containing IL-6 (35 ng/mL) or MDA-SFCM, and then media were replaced with complete medium and cells were passaged several times. A. Cell lysates were prepared and were used for immunoblotting analysis using specific antibodies and GAPDH was used as internal control. B. Total RNA was prepared from the indicated cells, and then Let-7b levels were assessed by qRT-PCR. Error bars indicate mean +/− SD of three experiments. C. Cell lysates were prepared from breast stromal fibroblasts isolated from tumors (T) and their adjacent histologically normal adjacent tissues (N), and the levels of the indicated proteins were assessed by immunoblotting.