Figure 3. IL-6 activates the IL-6/STAT3/NF-κB feedback loop in an autocrine manner.

NBF-6 cells were treated as in Figure 2A, and then either anti-IL-6 inhibitory antibody or anti-IgG were added for 24 h, and then cells were subcultured in absence of the inhibitory antibodies. A. Cell lysates were prepared and were used for immunoblotting analysis. B. Cell proliferation, migration and invasion of the indicated cells were assessed using the RTCA-DP xCELLigence System.