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. 2016 Jun 7;7(27):42172–42182. doi: 10.18632/oncotarget.9902

Figure 4. ROS conferred increased miR-21 expression by LPS stimulation.

Figure 4

(A, B) Human lung cancer cells freshly isolated from different tissues (n = 3) were treated with or without LPS (10 μg/ml) for 24 h and detected for their ROS levels with flow cytometry. (C, D) Human lung cancer cells freshly isolated from different tissues (n = 3) were transfected with TLR4 shRNA or control shRNA, stimulated with LPS (10 μg/ml) for 24 h and detected for ROS generation. (E) Human lung cancer cells freshly isolated from different tissues (n = 3) were treated with LPS (10 μg/ml) in the presence or absence of Tempol (50 μM) for 24 h, and detected for their growth with MTT assay. (F) Human lung cancer cells freshly isolated from different tissues (n = 3) were treated with LPS (10 μg/ml) in the presence or absence of Tempol (50 μM) for 24 h, and assayed for their miR-21 expressions. (G) Human lung cancer cells freshly isolated from different tissues (n = 6) were treated with LPS (10 μg/ml) in the presence or absence of Tempol (50 μM) for 24 h, and injected into nude mice. Tumor volumes (mean ± SD) were determined from 6 mice per group.