Figure 4. GAPLINC accelerated the growth of CRC cells in vivo.
A, B. Photographs of the xenograft-transplanted nude mouse tumor models and the harvested tumors were captured 4 weeks after the injection of GAPLINC-shRNA or empty vector of HCT116 cells (n = 5). C. Tumor volume was calculated as the length × width2 × 0.5 every week after injection. D. Weights of xenografts were measured when the mice were sacrificed. E. qPCR analysis of GAPLINC expression in xenograft tumor tissues. F. Hematoxylin and eosin and immunohistochemical (IHC) staining of the xenograft tumors. IHC staining showed that Ki67 expression was weakened in the GAPLINC-shRNA group compared with the empty vector group. Data represent mean ± standard deviation from three independent experiments. *P < 0.05, **P < 0.01.