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. 2016 Jun 11;7(27):42261–42273. doi: 10.18632/oncotarget.9950

Figure 1. The expression of ERα is negatively correlated with LY6K.

Figure 1

(A and B) Expression of LY6K and ERα mRNA and protein was observed by qRT-PCR using specific primers and western blotting. h18s rRNA and β-actin were used for endogenous control. (C and D) Ectopic expression of LY6K in MCF7 and T47D downregulated ERα transcriptional activity and protein expression. (E and F) Ectopic expression of ERα in MCF7-ADR and MDA-MB-468 reduce LY6K mRNA and protein expression. Data are a mean ± S.D. (error bars) of three independent experiment in triplicate. ***p < 0.001; **p < 0.01; *p < 0.05.