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. 2016 Jun 11;7(27):42261–42273. doi: 10.18632/oncotarget.9950

Figure 2. The regulation of ERα and LY6K expression in a miRNA-dependent manner.

Figure 2

(A and B) Gene expression level was determined on both mRNA and Protein levels after treatment with Ago2 siRNA combined with overexpression of LY6K for 48 hr. The expression of mRNA and protein was analyzed by qRT-PCR and western blotting. (C and D) LY6K expression in response to knock-down of AGO2 by treating with control and ERα for 48 hr. The expression of mRNA and protein was investigated by qRT-PCR and western blotting. Data are a mean ± S.D. (error bars) of three independent experiment in triplicate. ***p < 0.001; **p < 0.01; *p < 0.05.