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. 2016 Jun 11;7(27):42261–42273. doi: 10.18632/oncotarget.9950

Figure 3. Validation of primary and mature miRNAs induced by LY6K or ERα.

Figure 3

(A) Validation of upregulated primary miRNA (pri-miRNA) expression in T47D/LY6K was compared with T47D/Mock (left) and MCF7-ADR/Mock compared with MCF7-ADR/ERα (right) by using qRT-PCR. Each primary miRNA produces mature miRNAs (Pri-miR-29a/b1: miR-29b, Pri-miR29b2/c: miR-29b and miR-29c, Pri-miR-194-2/192: miR-192-5p and miR-194, Pri-miR-194-1: miR-194, Pri-miR-34a: miR-34a, Pri-miR-500a: miR-500a-5p or -3p). (B) Verification of miRNA expression induced by LY6K in ERα-positive breast cancer cell lines, MCF7 (left) and T47D (right). (C) Verification of mature miRNA induced by ERα in ERα-negative breast cancer cell lines, MCF7-ADR (left) and MDA-MB-468 (right). Data are a mean ± S.D. (error bars) of three independent experiment in triplicate. ***p < 0.001; **p < 0.01; *p < 0.05.