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. 2016 Jun 11;7(27):42261–42273. doi: 10.18632/oncotarget.9950

Figure 4. miR-192-5p directly binds ERα 3′UTR and regulates its expression.

Figure 4

(A) Gene structure of ESR1 showing the predicted target site of miR-192-5p in its 3′-UTR. (B) Luciferase assay in MCF7 cells showed miR-192-5p dependent repression of wild type (WT) ERα 3′UTR (left), whereas mutation (MT) of the miR-192-5p predicted binding site did not affect repression (right). (C) Re-expression of miR-192-5p repressed the mRNA and protein expression of ERα in MCF7 and T47D. (D) mRNA and Protein expression of LY6K was determined in Human LY6K over-expressing stable cell line by qRT-PCR and western blot. (E) Inhibition of miR-192-5p expression increases ERα expression in T47D/LY6K stable cells. Mimic, miR-192-5p mimic treated cell; inhibitor, miR-192 inhibitor treated cell. Data present mean ± S.D. (error bars) of three independent experiments in triplicate. ***p < 0.001; **p < 0.01.