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. 2016 Jun 11;7(27):42261–42273. doi: 10.18632/oncotarget.9950

Figure 5. LY6K is a direct target of miR-500a-3p and down regulates its expression.

Figure 5

(A) Gene structure of LY6K showing the two predicted target sites of miR-500a-3p in its 3′UTR. (B) Dual Luciferase assay in HEK293T cells showed ERα (left) and miR-500a-3p (right) dependent inhibition of wild type (WT) LY6K 3′-UTR, whereas the mutant type (MT) seed sequence restored luciferase activity. (C) Ectopic expression of miR-500a-3p represses LY6K mRNA and protein expression in MCF7-ADR (left) and MDA-MB-468 (right). NC, miRNA negative control; mimic, miRNA mimic. Data represent mean ± S.D. (error bars) of three independent experiment in triplicate. ***p < 0.001.