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. 2016 Jun 2;7(27):42314–42329. doi: 10.18632/oncotarget.9791

Figure 6. Direct transdifferentiation of human fibroblasts into corneal epithelial lineage.

Figure 6

A. Bright field images of transdifferentiated fibroblasts 7 days post infection either with PCT, PCTK, PCTO or PCTOK (explained in the text). Scale bar: 200 μm. B. Immunocytochemistry analysis of transdifferentiated fibroblasts infected with PCT, PCTK, PCTO or PCTOK for the expression of CK3 and CK12. Cells were analyzed on day 7 post infection. Scale bar: 100 μm. C. RT-PCR for transduction efficiency analysis. 1st band: transfection of HEK-293 for virus production. 2nd band: transduction of human dermal fibroblasts with virus. Abbreviations: PCT, lentiviral cocktail containing ΔNp63, C/EBPδ and TCF4 lentiviruses; PCTK, lentiviral cocktail containing PCT plus Klf4; PCTO, lentiviral cocktail containing PCT plus Oct4; PCTOK, lentiviral cocktail containing PCT plus Oct4 and Klf4. D. Analysis of CK3 and CK12 fluorescence intensities for transdifferentiated fibroblasts infected with PCT, PCTK, PCTO or PCTOK. Results are presented as the percentage of PCT intensity ± SD.