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. 2016 Jun 6;7(27):42340–42352. doi: 10.18632/oncotarget.9851

Figure 4. Effect of EGF on cell proliferation, ErbB1, ErbB2 and their downstream signaling molecules.

Figure 4

A. Cells were treated by solvent or EGF as indicated, followed by measurement of cell density by the MTT assay. Each value is mean ± SD (n=3). ****P<0.0001. B. Cells were untreated or treated by EGF as indicated, from which cell lysates were prepared and analyzed by IB. Measurement of protein phosphorylation includes p-Y1173-ErbB1, p-Y1221/1222-ErbB2, p-T202/Y204-ERK and p-S473-AKT.