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. 2016 Jun 7;7(27):42408–42421. doi: 10.18632/oncotarget.9878

Figure 1. Effects of CPT fluorescence across tight-junction barrier and cell membranes following CRLX101 treatment.

Figure 1

Tight-junction barrier was constructed by CECs. CRLX101 (30 μg/ml) was added to the upper chamber for the indicated time intervals. The permeability of the CEC monolayer was determined by a TEER assay after 24 hours A. The bottom medium was collected to measure the fluorescence of CPT. Concentrations were determined by comparison to a CRLX101 standard curve B. Each value represents the mean ± SE for n=3. *, p<0.05, compared with respective control. Mice were intravenously injected with 10 mg/kg CRLX101 for various time intervals. CPT fluorescence of was evaluated, and concentrations in the plasma C. and brain D. were quantified with a CRLX101 standard curve. Each value represents the mean ± SE for n=3. U87 MG cells were treated with CPT or CRLX101 for 6 hours and stained with Mitotracker Red. The fluorescence was detected using a deconvolution microscope E. Data shown are representative fluorescent micrographs of three independent experiments. Scale, 10 μm.