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. 2016 Jun 7;7(27):42431–42446. doi: 10.18632/oncotarget.9883

Figure 2. Effect of HULC on HCC cell growth in vitro.

Figure 2

A. Analysis of HULC expression levels in HCC cell lines (Huh-6, Huh-7, HepG2, BEL-7402, MHCC-97H, Sk-Hep1 and SMMC-7721) compared with L02 cell as determined using qRT-PCR and normalized to GAPDH expression. B. Relative expression levels of HULC in Huh-6, BEL-7402 and SMMC-7721 cells transfected with siRNA-NC or HULC siRNA, as determined using qRT-PCR. C. CCK8 was performed to determine cell viability and proliferation of Huh-6, BEL-7402 and SMMC-7721 cells. D. Representative results of colony formation of Huh-6, BEL-7402 and SMMC-7721 cells transfected with siRNA-NC or HULC siRNA. E. Huh-6, BEL-7402 and SMMC-7721 cells were stained and analyzed by flow cytometry after transfection for 24 hours. Values shown indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.