Skip to main content
. 2016 Jun 7;7(27):42431–42446. doi: 10.18632/oncotarget.9883

Figure 6. HULC enhanced epithelial to mesenchymal transition by sequestering miR-200a in HCC cells.

Figure 6

A. Western blot was used to measure expression levels of ZEB1 and E-Cadherin in Huh-6, BEL-7402 and SMMC-7721 cells transfected with miR-200a-3p mimics or miR-NC, and normalized to β-actin expression. B. The effect of increasing miR-200a-3p expression in cell invasion and migration of Huh-6, BEL-7402 and SMMC-7721, transfected with miR-200a-3p mimics or miR-NC, as determined using a transwell assay (magnification, 100×, scale bars=50 μm). C. Expression levels of ZEB1 and E-Cadherin as determined using western blot in Huh-6, BEL-7402 and SMMC-7721 cells transfected with the miR-200a-3p inhibitor, miR-NC or HULC siRNA, and normalized to β-actin expression. D. Expression levels and location of ZEB1 and E-Cadherin as determined using immunocytochemistry in Huh-6, BEL-7402 and SMMC-7721 cells transfected with miR-200a-3p mimics, miR-NC or HULC siRNA (magnification, 400×, scale bars=20 μm).