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. 2016 Jun 10;7(27):42566–42578. doi: 10.18632/oncotarget.9934

Figure 1. Down-regulation of KLF4 by TGF-β1.

Figure 1

A. and B. Bel-7402 cell was starved for 24h and then treated with TGF-β1 in various concentrations (0-12.5ng/ml) for 24h as indicated. Expression of KLF4 protein level was examined (A) and mRNA level was examined by qRT-PCR (B). The relative mRNA levels were normalized to β-actin and presented as mean ± SEM, with each experiment conducted in triplicate (*, P<0.05; **, P<0.01, n=3). C. and D. SK-Hep-1, Hep-3B and Huh 7 cells were starved for 24h and then treated with TGF-β1 (10ng/ml) for 24h. The expression of KLF4 protein levels and mRNA levels were examined, respectively (**, P<0.01; ***, P<0.001, n=3). E. HCC cells were starved for 24h and then treated with TGF-β1 (10ng/ml) for 24h. The expression of E-cadherin mRNA level was examined by qRT-PCR (*, P<0.05; **, P<0.01, n=3).