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. 2016 Jun 10;7(27):42566–42578. doi: 10.18632/oncotarget.9934

Figure 2. Up-regulation of MicroRNA-135a-5p by TGF-β1.

Figure 2

A. Hep-3B and Bel-7402 cells were transfected with selected miRNAs mimics at each 100nM concentration (miR-206, miR-26b-5p, miR-214-3p, miR-375, miR-449a, miR-135a-5p, miR-9-5p, miR-107, miR-128-1-5p, miR-363-3p, miR-367), respectively. 48h after transfection, cells were harvested and qRT-PCR analysis of KLF4 normalized to β-actin was performed, data were presented as mean ± SEM (*, P<0.05, n=3). B. Hep-3B cell was starved for 24h and then treated with TGF-β1 (10ng/ml) for another 24h. The expression of 11 miRNAs as indicated were measured by qRT-PCR analysis normalized to U6 snRNA (**, P<0.01, n=3). C. SK-Hep-1, Hep-3B and Huh 7 cells were starved for 24h and then treated with TGF-β1 (10ng/ml) for another 24h. The expression of miR-135a-5p was measured by qRT-PCR analysis normalized to U6 snRNA (*, P<0.05, n=3). D. HCC cells were starved for 24h and then treated with TGF-β1 (10ng/ml) for another 24h. The expression of Pri-miR-135a-5p normalized to β-actin was examined by qRT-PCR (*, P<0.05; **, P<0.01; ***, P<0.001, n=3).