Skip to main content
. 2016 Jun 14;7(27):42585–42597. doi: 10.18632/oncotarget.10018

Figure 2. pY36 facilitates coactivator-dependent ubiquitylation and turnover of ERβ.

Figure 2

A. Ectopic expression of p300 promoted ligand-dependent transcriptional activation of MDA7 by ectopic ERβ in HEK293T cells. B. siRNA-mediated p300 knockdown blunted ectopic ERβ activity in HEK293T cells. C. In vitro ubiquitination assay containing E1/E2 ubiquitin ligases, purified bacterially expressed His-ERβ, bacterially expressed GST-p300(1-595) or mammalian cell-expressed myc-p300(1-595). D. p300 knockdown substantially reduced the extent of ERβ ubiquitination in HEK293T cells.