Fig 3. Effect of Vaccination Route and the Dose of Adjuplex and OVA on the Magnitude of CD8 T-cell Responses.

(A) Intramuscular and subcutaneous vaccination with increasing doses of Adjuplex generate comparable primary CTL responses. WT C57BL/6 mice were vaccinated by intramuscular or subcutaneous injection of 10 μg ovalbumin in PBS alone or mixed with 5, 10 or 20% Adjuplex. At day 7 after vaccination, percentages of SIINFEKL-specific IFN-γ⁺ CD8 T cells in spleen were determined by intracellular staining. (B) Primary OVA-specific CTL responses in the spleen are proportionate to SQ vaccination with increasing doses of Adjuplex and OVA. C57BL/6 mice were vaccinated by subcutaneous injection of 1, 3, or 10 μg ovalbumin in PBS alone or supplemented with 1,3, 5 or 10% Adjuplex. The bar graph represents the frequency of SIINFEKL-specific IFN-γ⁺ CD8 T cells among all CD8⁺ splenocytes after vaccination at each dose. Data are from analysis of 3–4 mice/group and are representative of two independent experiments.