Fig 2. Ganglioside profile of the retina and other ocular tissues, brain and plasma.

A. Resorcinol-stained HPTLC plate of GGs extracted from retina, retinal pigment epithelium (RPE)/choroid, ciliary body, optic nerve, brain and plasma. GG aliquots (4–7 independent samples) were pooled for each tissue type to represent a total of 10 nmol GG-sialic acid spotted per lane. A standard mixture of ganglio-series GGs (Std, 5 nmol sialic acid) was also spotted. The plate was developed in CHCl₃/CH₃OH/0.2% CaCl₂ (55:45:10, v/v/v) and revealed with resorcinol reagent. B. Quantitative distribution of GG classes calculated from a standard curve of each GG class. Results are expressed in nmol GG/mg protein or nmol GG/mL plasma.