Fig. 5.
Effect of various intracellular inhibitors on FGF9-induced CCND1 (Panel A) and CDK4 (Panel B) mRNA expression in large-follicle TC (Exp. 5). Cells were cultured for 72 h as described in Materials and Methods, and then treated for an additional 24 h with IGF1 (30 ng/mL) plus and FGF9 (30 ng/mL) and either LY294002, H89, U0126 or wortmannin. Values are normalized to constitutively expressed 18S ribosomal RNA and are means ± SEM of three separate experiments. a,b,cWithin a panel, means without a common letter differ (p < 0.05).