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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Immunol. 2016 Nov 28;198(1):352–362. doi: 10.4049/jimmunol.1600914

Figure 6. Treg depletion is accompanied by shift toward increased M2.

Figure 6

Systemic depletion of Tregs following DT treatment of DTR transgenic mice. (A) Representative FACS plots, (B) distribution and (C) absolute number and fold change of IM/M1 and M2 one day following final DT treatment. (D) Effect of Treg depletion on skeletal muscle M1 and M2 proliferation (Ki67+) one day following final DT treatment. (A-D) Results are representative of n = 10 per group from four experiments. (E) Gating scheme for Ly6chi and Ly6clow monocytes. Frequencies and absolute numbers of (F) bone marrow and (G) blood Ly6chi and Ly6clow monocytes (CD45+TCRβ-Ly6G-SiglecF-NK1.1-TER119-CD11b+CD115+) one day following final DT treatment. Results are representative of n = 13 per group from three experiments; error bars are the SD. *P < 0.05; **P < 0.01; (C, E, F) Kruskal-Wallis test, (D-F) Student's t test.