Figure 7. Treg ablation rescues skeletal muscle fiber regeneration.

(A) Representative H&E stains of hindlimb muscles one day following final DT treatment in DTR^- and DTR⁺ mice 28 dpi. Arrows indicate centrally nucleated myofibers. (B) Quantification of regenerating fibers (centrally nucleated myofibers) in muscle sections per mm² (C) Fold expression of myogenic targets MyoD and myogenin in infected DTR⁺ skeletal muscle normalized to DTR^- skeletal muscle following DT treatment Statistics were calculated on log-transformed fold change values. (D) qRT-PCR quantification of total parasite burden by B1 and (E) parasite stage specific transcripts Bag1 bradyzoite (left) and Eno2 tachyzoite (right) following DT treatment. Results are representative of n = 10 per group from four experiments; error bars are the SD. *P < 0.05; Student's t test