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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Immunol. 2016 Nov 16;198(1):318–334. doi: 10.4049/jimmunol.1502381

Fig. 4. Dissolution of NETs by DNase I disrupts early, but not late, neutrophil clusters. Neutrophil clusters form in the absence of intact NET structures.

Fig. 4

(A) Human neutrophils assayed as in Fig. 2 on Fn + β-glucan. DNase I was added either at time 0 before any neutrophil clusters had formed, after 10 min when early clusters had formed and associated NETs could be visualized, or after 25 min with matured clusters and associated NETs could be visualized. After 30 min incubation, cluster formation and NETs in the presence or absence of DNase I was visualized using Sytox green and imaged. Bright field and FITC images acquired at 20x; bar = 100μm.