Table 1.

One-pot three-enzyme (OP3E) preparative-scale synthesis of α2–8-linked sialosides. Yields were calculated for purified products based on the amounts of acceptor (the limiting reagent) used.

Entry	Sia Precursor	Acceptor	Sialosides (Siaα2–8Neu5Acα2–3GalβpNP)	Yield
a		A1		85%
b		A1		90%
c		A1		92%
d		A1		82%
e		A1		95%
f		A1		85%
g		A1		86%