Figure 1. Loss of Raptor results in a reduction of AMΦ pool in adult mice.

(A) Representative dot plots of CD45⁺ living cells from single cell preparations of enzymatically digested lung from adult (6–9 weeks old) WT and Rptor^ΔMyel mice.

(B) Frequencies and absolute numbers of AMΦ from 6–16 weeks old WT and Rptor^ΔMyel mice. Each circle or square represents on mouse of the indicated genotypes. Data are expressed as mean ± SEM; n ≥ 9 per group.

(C) Representative dot plots of CD45⁺ living cells from single cell preparations of enzymatically digested lung from WT and Rptor^ΔMyel mice at the age of 7 days.

(D) Frequencies and absolute numbers of AMΦ from WT and Rptor^ΔMyel mice at the age of postnatal day 7. Data are expressed as mean ± SEM; n ≥ 4 per group. n.s., non-significant.

(E) Quantitative real-time PCR analysis of PU.1 and PPAR-γ mRNA in sorted AMΦ; results are presented relative to those of the control gene β-actin. Data are shown as mean ± SEM of triplicates and are representative of two independent experiments.

(F) Quantification of macrophages from various tissues, including interstitial macrophages (IMΦ) of the lung, large peritoneal macrophages (LPΦ), small peritoneal macrophages (SPΦ), spleen red pulp macrophages (RPΦ), and bone marrow macrophages (BMΦ). Data are expressed as mean ± SEM; n ≥ 5 per group.

(G – I) Zsgreen reporter expression was analyzed in macrophages with indicated genotypes. Representative histograms show Zsgreen levels in AMΦ from adult mice (G) and 7 days old mice (H). (I) Zsgreen⁺ cells among macrophages from various tissues in adult mice. Data are expressed as mean ± SEM; n ≥ 2 per group.

(J) Indicated leucocytes counts in the lung from WT and Rptor^ΔMyel mice. Data are expressed as mean ± SEM; n ≥ 3 per group.