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. Author manuscript; available in PMC: 2017 Jan 27.
Published in final edited form as: Oncogene. 2016 Jun 20;36(4):534–545. doi: 10.1038/onc.2016.224

Figure 10. ANF inhibits OSC tumor growth and nuclear YAP1 in vivo.

Figure 10

A. Growth of OSC tumors is inhibited by ANF. OVCAR3 cells were injected subcutaneously into the flanks of immuno-deficient mice and allowed to establish palpable tumors as described in Materials and Methods. Beginning on day 32, tumor-bearing animals were randomly divided into two groups receiving either ANF (12 mg/kg) or saline daily. Tumor size was monitored by caliper measurements as described. Tumor size (B) and weight (C) were determined at the time of sacrifice (day 68). Results represent mean +/−SEM. N=12. *p<0.05 compared to vehicle control. D. Immunohistochemistry for YAP1 of representative tumors from DMSO and ANF-treated mice. E. Quantitative analysis of YAP1 immunohistochemistry was conducted as described in Materials and Methods. *p<0.05 compared to DMSO-treated tumors using Chi-Square analysis. F. Immunohistochemistry for YAP1 and PKCι from representative primary OPSC tumors. G. Analysis of IHC of primary OSC tumors for PKCι and YAP1 reveals a positive correlation between PKCι expression and nuclear YAP1.