Skip to main content
. Author manuscript; available in PMC: 2017 Jan 27.
Published in final edited form as: Oncogene. 2016 Jun 20;36(4):534–545. doi: 10.1038/onc.2016.224

Figure 3. YAP1 is required for transformed growth of OSC cells.

Figure 3

A. Immunoblot analysis of NT, and YAP1 KD OSC cells expressing either control vector (V), wild-type YAP1 (WT) or a constitutive active WW domain YAP1 mutant (WW) for YAP1. Actin serves as a control for loading. B. Effect of YAP1 KD and reconstitution with WT or WW mutant YAP1 on soft agar colony formation. Results are expressed as % NT control +/−SEM. N=5. *p<0.05 compared to NT; **p<0.05 compared to YAP1 KD. C. Effect of YAP1 KD and reconstitution with WT or WW mutant YAP1 on clonal expansion. Results are expressed as % clonal expansion. *p<0.05 compared to NT; **p<0.05 compared to YAP1 KD by Chi-square analysis. N=25 (OVCAR3) and 30 (OAW28). D. QPCR analysis for expression of YAP1, CYR61, ANKRD1, CTGF and MMP10. Results expressed as % NT control. N=3. *p<0.05 compared to NT; **p<0.05 compared to YAP1 KD.