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. Author manuscript; available in PMC: 2017 Jan 27.
Published in final edited form as: Oncogene. 2016 Jun 20;36(4):534–545. doi: 10.1038/onc.2016.224

Figure 8. PKCι-mediated phosphorylation regulates AMOT130 binding to YAP1 and transformed growth of OSC cells.

Figure 8

A. OAW28 cells transfected with Flag-tagged WT-AMOT130, T750A AMOT130, T750D-AMOT130 or empty vector were subjected to immunoprecipitation using FLAG antibody followed by immunoblot analysis for YAP1 and FLAG. YAP1 and GAPDH immunoblot of input served as loading control. B. Quantitative analysis of binding of YAP1 to AMOT mutants. Results represent YAP1 bound to the indicated AMOT mutant expressed as fold of YAP1 bound to WT-AMOT. Data represent the mean +/− SEM. N=3. *p<0.05 compared to WT. C. Soft agar colony formation in OAW28 cells transfected with the indicated expression vector. Results are expressed as percent of vector control and represent the mean +/− SEM. N=3. *p<0.05 compared to vector. **p<0.05 compared to WT. D. Effect of AMOT130 mutants on clonal expansion of OAW28 cells in non-adherent culture. Representative micrographs are shown. E. Quantitative analysis of clonal expansion of OAW28 cell transfectants. Results are expressed as percent of total cells expanded. *p<0.05 compared to Vector control. **p<0.05 compared to WT using Chi-square analysis. F. Immunoblot analysis of OAW28 cell transfectants for CYR61. GAPDH serves as a loading control.