Table 4.
Phylogenetic affiliations and number of bacterial clones obtained from retained and suspended fractions in the MFC operated for 30 days at pH 7.0
| OTUa | No. of clonesb | Phylogenetic group | The closest isolated strain (accession no., similarity) | The closest environmental clone (accession no., similarity) | Isolated source of environmental clones | |
|---|---|---|---|---|---|---|
| Retained | Suspended | |||||
| 1 | 6 | Proteobacteria; Deltaproteobacteria |
Geobacter sulfurreducens
(NR_075009, 98.6%) |
Uncultured bacterium (GQ152932, 100%) |
Mixed consortium in MFC | |
| 2 | 4 | Proteobacteria; Gammaproteobacteria |
Citrobacter freundii
(AB548830, 100%) |
Uncultured organism (HQ745055, 99.9%) |
Gastrointestinal specimens | |
| 3 | 3 | 1 | Proteobacteria; Gammaproteobacteria |
Raoultella ornithinolytica
(CP004142, 100%) |
Uncultured bacterium (EF515418, 100%) |
Upflow MFC anode |
| 4 | 4 | 1 | Proteobacteria; Gammaproteobacteria |
Pseudomonas fragi
(AB685617, 99.8%) |
Uncultured bacterium (EU469649, 99.8%) |
Mammalian gut microbes |
| 5 | 5 | Proteobacteria; Gammaproteobacteria |
Aeromonas dhakensis
(KF938660, 100%) |
Uncultured bacterium (JX271966, 100%) |
Activated sludge from large-scale | |
| 6 | 3 | Proteobacteria; Gammaproteobacteria |
Aeromonas sharmana
(NR_043470, 96.9%) |
Uncultured Aeromonas sp. (EF679186, 98.6%) |
Sewage plant Anaerobic sludge from MFC |
|
| 7 | 1 | Proteobacteria; Betaproteobacteria |
Advenella kashmirensis
(KF956701, 95.1%) |
Uncultured bacterium (FN563161, 99.7%) |
Mesophilic biogas digester | |
| 8 | 1 | 1 | Proteobacteria; Betaproteobacteria |
Azospira oryzae
(DQ863512, 94.1%) |
Uncultured Azospira sp. (JF736645, 96.5%) |
Biofilm on electrode in MFC |
| 9 | 12 | 5 | Bacteroidetes; Cytophagia |
Pontibacter korlensis
(GQ503321, 82.7%) |
Bacteriodetes bacterium (JX828412, 95.9%) |
Iron-reducing enrichment culture |
| 10 | 3 | Bacteroidetes; Bacteroidia |
Bacteroides nordii
(NR_112939, 92.9%) |
Uncultured bacterium (KC179060, 99.7%) |
Activated sludge | |
| 11 | 12 | Bacteroidetes; Bacteroidia |
Dysgonomonas oryzarvi
(NR_113063, 99.7%) |
Uncultured Dysgonomonas sp. (JX535216, 100%) |
Activated sludge from sewage | |
| 12 | 1 | Bacteroidetes; Bacteroidia |
Dysgonomonas capnocytophagoides
(NR_113133, 93.2%) |
Uncultured bacterium (HQ728219, 96.1%) |
Glucose-fed MFC | |
| 13 | 3 | Firmicutes; Negativicutes |
Anaeroarcus burkinensis
(NR_025298, 97.7%) |
Uncultured bacterium (AB329655, 99.9%) |
UASB granule | |
| 14 | 2 | Firmicutes; Negativicutes |
Acidaminococcus fermentans
(NR_074928, 93.8%) |
Uncultured bacterium (JN680060, 99.5%) |
Anode biofilm at psychrotolerants in MFC | |
| 15 | 1 | Firmicutes; Clostridia |
Pseudoflavonifractor capillosus
(NR_025670, 89.8%) |
Uncultured Clostridiales bacterium (FJ393118, 91.0%) |
Glucose-fed MFC anode | |
| Sum | 39 | 30 | ||||
aSequences with more than 97.0% homology were considered as being the same as the operational taxonomic unit (OTU)
bSuspended: the suspended fraction obtained from fermentation broth, Retained: the retained fraction obtained from anode graphite felts