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. 2004 Aug 1;18(15):1824–1837. doi: 10.1101/gad.1223504

Figure 2.

Figure 2.

Prx I induction by arsenate is enhanced in c-Abl-deficient osteoblasts. (A) Induction of Prx I under different concentrations of sodium arsenate in abl-/-, c-Abl reconstituted abl-/-, and control calvarial osteoblasts. Equal numbers of osteoblasts were plated and treated with different amounts of sodium arsenate for 10 h, and Prx I mRNA levels were analyzed by Northern blot. (B) Quantitation data from three repeated experiments. (C) Western blot analysis showing that expression of retroviral-driven c-Abl is slightly higher than that of endogenous levels. (D) Up-regulation of Nrf2 was enhanced in c-Abl-deficient osteoblasts. Mutant and control osteoblasts were treated with different concentrations of arsenate for 6 h and collected, and Western blot analysis was carried out to test the expression of Nrf2. (E) c-Abl-deficient osteoblasts are hypersensitive to the cytotoxicity of arsenate. Cell death rates in abl-/-, reconstituted abl-/-, and wild-type control osteoblasts induced by arsenate. The values are mean ± S.D. (n = 4).