Figure 5.

Osteoblasts deficient for p53 showed a normal response to arsenate treatment. (A) Induction of Prx I by arsenate in p53^-/- and control osteoblasts. Osteoblasts isolated from p53 knockout mice and their control littermates were stimulated with different concentrations of arsenate for 10 h. Total RNA was isolated from these cells and analyzed by Northern blot to compare the expression levels of Prx I. (B) Cell death rates under arsenate treatment. Osteoblasts deficient for p53 and their control cells were treated with 0.10 or 0.15 mM of arsenate for 16 h and the percentage of dead cells was determined by the trypan blue exclusion method.