Figure 5.

Morphological analysis of wild-type, ΔrecQ, dnaE486, and dnaE486 ΔrecQ mutant cells. (A) Cells were grown to early log phase at 30°C, and then grown for 6 h at 38°C. Cells were stained with DAPI (4′,6′-diamidino-2-phenylindole) and observed under a phase contrast microscope. White arrowheads highlight some anucleate cells. (B) Distribution of cell sizes. Cells grown for 6 h at 38°C were stained with DAPI. The shapes and chromosomal DNA were monitored under a microscope. Proportions of normal cells are indicated by white bars. Slightly (gray bars) and highly (black bars) filamented cells are defined as cells larger than three and 10 cell lengths in size regardless of the number of nucleoids, respectively. At least 300 cells were examined.