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. 2004 Aug 1;18(15):1898–1907. doi: 10.1101/gad.1209404

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Figure 2. — Effect of GalR on gal promoters in vitro. Results of in vitro transcription at varying concentrations of GalR (A–D). Plasmid DNA template (pSA850) with GalR⁺ (A), and GalR^T322R (B). Plasmid DNA template (pSA886) with GalR⁺ (C) and GalR^T322R (D). Quantification of activity of P1 (E) and P2 (F) at varying concentrations of GalR. RNA1 transcript, which does not vary with GalR concentration, was used as an internal control between lanes. Promoter activities in the absence of GalR were used as a reference (1.00) in each experiment. Plasmid DNA template (pSA850) with wild-type GalR (black squares), and GalR^T322R (red dots). Plasmid DNA template (pSA886) with wild-type GalR (green triangles) and GalR^T322R (blue triangles).

Inline graphic — Effect of GalR on gal promoters in vitro. Results of in vitro transcription at varying concentrations of GalR (A–D). Plasmid DNA template (pSA850) with GalR⁺ (A), and GalR^T322R (B). Plasmid DNA template (pSA886) with GalR⁺ (C) and GalR^T322R (D). Quantification of activity of P1 (E) and P2 (F) at varying concentrations of GalR. RNA1 transcript, which does not vary with GalR concentration, was used as an internal control between lanes. Promoter activities in the absence of GalR were used as a reference (1.00) in each experiment. Plasmid DNA template (pSA850) with wild-type GalR (black squares), and GalR^T322R (red dots). Plasmid DNA template (pSA886) with wild-type GalR (green triangles) and GalR^T322R (blue triangles).