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. 2016 Dec 21;7:599. doi: 10.3389/fimmu.2016.00599

Figure 1.

Figure 1

(A) Binding of human C1q and recombinant globular head modules, ghA, ghB, and ghC (10 µg/ml; 1 h incubation) to SKOV3 cells using immunofluorescence microscopy. Panel A shows the nucleus of the cells stained with Hoechst. Panel B shows the cells probed with anti-C1q (C1q) and anti-maltose-binding protein (MBP) (globular heads) polyclonal antibodies, followed by anti-rabbit IgG labeled with FITC; the bound proteins are visible on the cell membrane (panels C and D). (B) Flow cytometric analysis to show binding of human C1q and ghA, ghB, and ghC (10 µg/ml) to SKOV3 cells after 1 h incubation. Panel a shows the number of cells probed with anti-C1q (C1q) and anti-MBP (globular heads) antibodies followed by anti-rabbit IgG labeled with FITC, as compared to the untreated cells. Panel b shows the shift in the fluorescent intensity from untreated to treated cells.