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Analysis of apoptosis using immunofluorescence microscopy in SKOV3 cells treated with human C1q, ghA, ghB, and ghC (10 µg/ml) and an untreated control after 24 h. Panel A shows the nucleus stained with Hoechst. Panel B shows the cell membrane integrity marker FITC Annexin V, which binds to PS of the cell membrane of cells undergoing apoptosis. No FITC was detected in the untreated SKOV3 cells.
