Figure 7.

Caspase activation in SKOV3 cells following treatment with C1q and globular head modules. (A) Western blot analysis of full-length/total caspase 3 at 32 kDa after 12 and 24 h of the treatment with C1q, ghA, ghB, ghC, and untreated. (B) The cleaved caspase 3 was observed at 17 kDa after 24 h of treatment with C1q, ghA, ghB, ghC, and untreated (C) Western blot analysis for β-actin as a loading control for 12 h and 24 h at 45 kDa. (D) The activation of caspase 3 was also shown by immunofluorescence microscopy at 24 h in parallel with apoptosis staining for Annexin V-FITC, where activated caspase 3 was clearly visible in the cytoplasm probed with CY3 at 24 h.