FIG. 3.

TLR-dependent induction of NF-κB-mediated transcription in bronchial epithelial cells stimulated with P. aeruginosa lipopeptide. 9/HTEo⁻ (A) and NuLi-1 (B) cells (both derived from normal human lung cells) were transiently cotransfected with an NF-κB-responsive luciferase reporter plasmid and TLR2 or DN-TLR2 and then incubated for 6 h in the presence of 10 μg of lipopeptide (LptA)/ml. The data were normalized to a cotransfected β-galactosidase construct and are means ± standard deviations. (C) Western blot analysis of TLR2 protein expression in NuLi and 9/HTEo⁻ cells with an anti-hTLR2 monoclonal antibody (IMG-319). Equal amounts of proteins were loaded. *, P < 0.05.