Table 1.

The CI of a GSE treatment combined with various anti-HCV agents on anti-HCV replication.

Combination compound	CI values at			Influence
	ED50	ED75	ED90
IFN-α	0.73	0.49	0.38	Synergistic
Telapriver	0.82	0.68	0.56	Synergistic
Daclatasvir	0.86	0.72	0.53	Synergistic
Sofobuvir	0.54	0.43	0.34	Synergistic

Ava5 cells were treated with GSE combined with IFN-α, telaprevir, daclatasvir, or sofosbuvir in a fixed ratio for 3 days. The combination treatment of anti-HCV activity was analyzed by qRT-PCR. The CI value for the effective dose of 50% (ED₅₀), 75% (ED₇₅), or 90% (ED₉₀) inhibition was calculated by using the CalcuSyn computer program. Results are expressed as the mean ± SD for three independent experiments. The calculated CI values indicated that CI < 1, CI = 1, and CI > 1 represented synergism, additivity, and antagonism on anti-HCV activity between the two agents, respectively.