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FPOP-MS work-flow. (i) Fast photochemical oxidation of proteins (FPOP) irreversibly labels solvent-accessible sites on protein side-chains; (ii) covalently modified proteins are digested to generate modified and unmodified tryptic peptides; (iii) LC-MS separation of these peptides is followed by (iv) MS/MS sequencing from which (v) a sequence can be derived for each peptide for identification of residue-specific modification sites. Modification sites are indicated by red circles
