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. 2016 Jun 24;28(1):50–55. doi: 10.1007/s13361-016-1421-1

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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LC-MS/MS analysis of the OmpT tryptic peptide T4 (residues 43-51) following FPOP. (a) Butterfly plot of LC-MS chromatograms showing unmodified T4 (blue peaks) in the absence of (upper trace) and following (lower trace) FPOP, and oxidized T4 (pink peak, lower trace, labelled “T4& FPOP”) following FPOP. U/A indicates an unassigned peak that was not identified as a modified or unmodified OmpT tryptic peptide; (b) and (c) show MS/MS spectra of unmodified and oxidized T4, respectively. The y” ions are labeled in red and the b ions in blue; the location of the modification site (Y44) is shown by the “Y&” symbol