Figure 3.

Tryptic peptides of OmpT (PDB 1I78 [29]) solubilized in DDM detergent micelles or in the amphipol A8-35 are modified to different degrees. Inset graphs show % peptide modified in DDM (blue) or in A8-35 (red) for four tryptic peptides of particular interest, and arrows (red and blue) indicate the respective residues that are modified in each peptide. Aromatic amino acid residues are shown in red. Residues towards the lower boundary of the transmembrane region are less readily labeled in DDM, whereas residues in the extra membrane region are shown to be less readily labeled in A8-35. Supplementary Figure S3 shows the location of the four tryptic peptides on the structure of OmpT