. 2016 Dec 7;2016:5695317. doi: 10.1155/2016/5695317

Table 1.

Primers and probes for the target SNP genotypes of SOX9 following LDR.

dbSNP	Primer^a	Probe^b	Product size^c
rs12601701 [A/G]	Sense: TCATACAACTAAGTACAGACG	TA:TTTTTTGACACAGAAATAGGTCCACACTACA	61/A,
rs12601701 [A/G]	Antisense: TGCATACAATAGCTTCGTATC	TG:TTTTTTTTTGACACAGAAATAGGTCCACACTACG TR:-P-CGGACTTTTTTCTCCTAGGAAAGGATTTTT-FAM	64/G

rs1042667 [A/C]	Sense: CACTGGGAACAACCCGTCTAC	TA:TTTTTTTTTTGAGGAGGCCTCCCACGAAGGGCGAA	68/A,
rs1042667 [A/C]	Antisense: TCCAAAGGGAATTCTGGTTGG	TC:TTTTTTTTTTTTTGAGGAGGCCTCCCACGAAGGGCGAC TR:-P-GATGGCCGAGATGATCCTAAAAATAACCGAAGA-FAM	71/C

^aPrimer to amplify the gene fragment of the target dbSNP site.

^bProbe to discriminate the target SNP genotype.

^cProduct size indicates the size of the ligase reaction product from the different SNP genotypes.