FIG. 5.
IL-12 was critical for optimal IFN-γ production on day 1. PBL with various number of mDCs were treated with or without 10 μg of anti-IL-12 neutralizing antibody/ml, followed by stimulation with 106 A. actinomycetemcomitans cells. The supernatants were collected after 24 h, and IFN-γ was measured by ELISA. Other controls included boiling the anti-IL-12 antibody for 30 min, which eliminated all suppressive activity. These data are representative of three separate experiments of this type and are expressed as means ± SE.