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. 2016 Dec 7;2016:4052846. doi: 10.1155/2016/4052846

Table 2.

Primers utilized in this study.

SNP Primers, 5′-3′ Product
Size (bp)
Function
rs2165870
(A/G)
F: TCCTAGGGCTCTGAAGAATTA 383 PCR
R: TTCAATTGACATTGCTCAGA
FA: TGCATCTGTTTACAGCCTTA 306 Genotyping
FG: TGCATCTGTTTACAGCCTTG

rs10802789
(C/T)
F: CTCGAAGTTAGGCGAGATTT 397 PCR
R: GTGTGACACGTGTAGGTCAA
FC: AAAGTGGCATTTCTCTACGC 213 Genotyping
FT: AAAGTGGCATTTCTCTACGT

rs685550
(C/T)
F: CCACATCAAACGTCGAGAGA 348 PCR
R: TTGAATCTATGCGCTTGTCG
FC: ATATAGAGATATTAAACAGC 241 Genotyping
FT: ATATAGAGATATTAAACAGT

rs7520974
(A/G)
F: TCTCCAAATCAACACTCCTG 302 PCR
R: CATGTCCTGTAGCACCATTT
FA: GCTGAAAGAAAGACAAATCA 175 Genotyping
FG: GCTGAAAGAAAGACAAATCG

rs3738435
(C/T)
F: CAGTGCATGCTACCAATTAT 361 PCR
R: TGTAGCTGACGAAATGAGTG
FC: TAAAATAAGAGAATGAACGC 289 Genotyping
FT: TAAAATAAGAGAATGAACGT

CHRM3RT F: ACCCAGCTCCGAGCAGATGGAC 339 RT-qPCR
R: CGGCTGACTCTAGCTGGATGGG
Probe: TGCCTGAGGAGGAGCTGCTGGGGA
TGGTGGACTTGGAGAG

SNP: single-nucleotide polymorphism; F: forward; R: reverse.