Table 2.
SNP | Primers, 5′-3′ | Product Size (bp) |
Function |
---|---|---|---|
rs2165870 (A/G) |
F: TCCTAGGGCTCTGAAGAATTA | 383 | PCR |
R: TTCAATTGACATTGCTCAGA | |||
FA: TGCATCTGTTTACAGCCTTA | 306 | Genotyping | |
FG: TGCATCTGTTTACAGCCTTG | |||
| |||
rs10802789 (C/T) |
F: CTCGAAGTTAGGCGAGATTT | 397 | PCR |
R: GTGTGACACGTGTAGGTCAA | |||
FC: AAAGTGGCATTTCTCTACGC | 213 | Genotyping | |
FT: AAAGTGGCATTTCTCTACGT | |||
| |||
rs685550 (C/T) |
F: CCACATCAAACGTCGAGAGA | 348 | PCR |
R: TTGAATCTATGCGCTTGTCG | |||
FC: ATATAGAGATATTAAACAGC | 241 | Genotyping | |
FT: ATATAGAGATATTAAACAGT | |||
| |||
rs7520974 (A/G) |
F: TCTCCAAATCAACACTCCTG | 302 | PCR |
R: CATGTCCTGTAGCACCATTT | |||
FA: GCTGAAAGAAAGACAAATCA | 175 | Genotyping | |
FG: GCTGAAAGAAAGACAAATCG | |||
| |||
rs3738435 (C/T) |
F: CAGTGCATGCTACCAATTAT | 361 | PCR |
R: TGTAGCTGACGAAATGAGTG | |||
FC: TAAAATAAGAGAATGAACGC | 289 | Genotyping | |
FT: TAAAATAAGAGAATGAACGT | |||
| |||
CHRM3RT | F: ACCCAGCTCCGAGCAGATGGAC | 339 | RT-qPCR |
R: CGGCTGACTCTAGCTGGATGGG | |||
Probe: TGCCTGAGGAGGAGCTGCTGGGGA TGGTGGACTTGGAGAG |
SNP: single-nucleotide polymorphism; F: forward; R: reverse.