FIG. 4.

LDAs with spleens from young and aged BALB/c mice 12 weeks after infection. We performed an LDA with the spleen from each (n = 5) young and senescent mouse in order to analyze visceralization. We did not detect parasites in the spleens of the three mice with healed ulcerations and detected only a very small number of parasites in another aged mouse, while only one senescent mouse showed marked dissemination into the spleen. In contrast, all of the young mice showed marked to very high dissemination of L. major into the spleen. *, P < 0.05 for differences between young and aged mice.