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. 2004 Sep;72(9):5106–5114. doi: 10.1128/IAI.72.9.5106-5114.2004

FIG. 8.

FIG. 8.

IL-12 secretion by macrophages from SPF facilities. Macrophages from mice kept under SPF conditions were stimulated for 24 h with LPS (20 ng/ml) as a positive control, with IFN-γ (100 U/ml), or with L. major opsonized with fresh mouse serum after priming with IFN-γ (100 U/ml). As shown for macrophages from senescent mice kept under conventional conditions, macrophages from senescent SPF mice also showed a slightly raised release of IL-12 compared to those from young mice, and this difference remained apparent after stimulation with L. major or IFN-γ. The secretion of IL-12 was stimulated by LPS in macrophages from aged and young mice. Data are means ± SD (n = 5). *, P < 0.05 for differences between young and aged mice.