TABLE 2.

Microbiological screening of mice from SPF and conventional facilities according to the criteria of FELASA by indirect immunofluorescence (IFA), cultures, and microscopic analysis

Target organions for test and test type	Method	Result for facility
		Conventional	SPF
Serology (viruses)
MHV	IFA	+++a	Negative
Ectromelia virus	IFA	Negative	Negative
Epizootic diarrhea of infant mice	IFA	Negative	Negative
Lymphocytic choriomeningitis virus	IFA	Negative	Negative
Mouse adenovirus 2 type K87	IFA	Negative	Negative
Murine cytomegalovirus	IFA	Negative	Negative
Mouse parvovirus	IFA	Negative	Negative
Lactic dehydrogenase virus	Enzymatic	Negative	Negative
Minute virus of mice	IFA	Negative	Negative
Pneumonia virus of mice	IFA	Negative	Negative
Reovirus type 3	IFA	Negative	Negative
Sendai virus	IFA	Negative	Negative
Theiler's encephalomyelitis virus	IFA	Negative	Negative
Polyomavirus	IFA	Negative	Negative
Hantaan virus	IFA	Negative	Negative
Mouse thymic virus	IFA	Negative	Negative
Bacteriology/mycology
Citrobacter rodentium	Culture	Negative	Negative
Clostridium piliforme	IFA	Negative	Negative
Corynebacterium kutscheri	Culture	Negative	Negative
Mycoplasma spp.	IFA	Negative	Negative
Pasteurella pneumotrophica	IFA	Negative	Negative
Streptobacillus moniliformis	Culture	Negative	Negative
Streptococcus sp. (B-hemolytic, not group D)	Culture	Negative	Negative
Streptococcus pneumoniae	Culture	Negative	Negative
Parasitology
Aspiculuris sp.	Microscopy	Negative	Negative
Syphacia sp.	Microscopy	Negative	Negative
Coccidia	Microscopy	Negative	Negative
Giardia sp.	Microscopy	Negative	Negative
Spironucleus muris	Microscopy	Negative	Negative
Trichomonada	Microscopy	Negative	Negative
Flagellates (other)	Microscopy	Negative	Negative
Arthropods	Stereomicroscopy	Negative	Negative

^a+++, high titers (>1:80).