FIG. 3.
B. burgdorferi DNA copy numbers in joint and ear tissues of control IgG- and RB6-treated mice. Mice were infected with 2.5 × 105 B. burgdorferi cells per footpad and treated daily with control IgG or RB6. On day 7 (A) or 21 (B and C), tibiotarsal joints (A and B) or ear samples (C) were collected, and the DNA was isolated and assessed by quantitative real-time PCR as described in Materials and Methods. Samples were normalized by using the single-copy mouse gene Nidogen, and the values are arithmetic means ± standard deviations for 103 single-copy mouse genes. The experiment was conducted twice with similar results by using three to five mice per group. An asterisk indicates that the P value is <0.05 for a comparison of IgG- and RB6-treated animals.